10 ENGLISH BOOKS RELATING TO «DIGITONIN»
Discover the use of
digitonin in the following bibliographical selection. Books relating to
digitonin and brief extracts from same to provide context of its use in English literature.
1
Mitochondria: Practical Protocols
Digitonin Fractionation of Neurospora crassa Mitochondria Many studies require
the localization of proteins or import intermediates to a distinct submitochondrial
compartment. A basic step in such fractionation is often the selective rupturing of
...
Dario Leister, Johannes Herrmann, 2007
2
GTPases Regulating Membrane Dynamics
Increasing digitonin concentrations extract outer membrane proteins as well.
Even higher digitcnin concentrations make holes in the mitochondrial inner
membrane, releasing mitochondrial matrix proteins, while the highest digitonin ...
William Edward Balch, Channing J. Der, Alan Hall, 2005
3
Exocytosis and Endocytosis
To permeabilize the plasma membrane, add digitonin-containing KHM buffer to
the cells. Determine the effective digitonin concentration by applying increasing
concentrations of digitonin to cells that express only GFP. GFP diffuses freely in ...
4
Subcellular Fractionation : A Practical Approach: A ...
Preparation of mitoplasts using digitonin Equipment and reagents • Isolation
buffer: 0.225 M mannitol, 0.075 M • High-speed and ultracentrifuge sucrose, 0.5
mM EGTA, 2 mM MOPS pH » Recrystallized digitonin 7.4 . Lubrol WX Method 1.
J. M. Graham, D. Rickwood, 1997
5
2-d Proteome Analysis Protocols
The 1X working digitonin extraction buffer should be prepared by adding solid
digitonin to a small amount of buffer and carefully boiling to dissolve. PMSF is
solubilized in the warm digitonin solution by slow addition with constant stirring ...
Several methods have been described; perhaps the easiest to use is the
digitonin extraction method, which follows (Fogshgaard et al., 2001). 6.1.
Digitonin extraction method Digitonin is a natural glycoside, which effectively
solubilize lipids, ...
Roya Khosravi-Far, Zahra Zakeri, Richard A. Lockshin, 2008
7
METHODS IN CELL BIOLOGY,VOLUME 31: VESICULAR TRANSPORT, PART ...
Digitonin at low concentrations (0.001%) apparently intercalates into the
membrane at cholesterol sites, weakening the membrane and causing
vesiculation, which generates small holes (Stearns and Ochs, 1982). The soluble
components of ...
Alan Michael Tartakoff, 1989
A digitonin-permeabilized cell system provided an in vitro assay for analyzing
soluble cytosolic components required for nuclear import (Figure 13-34).
Digitonin is a detergent that permeabilizes the plasma membrane, allowing
cytoplasmic ...
9
Biosynthesis and Biodegradation of Cellulose
SOLUBILIZATION OF CALLOSE AND CELLULOSE SYNTHASES A.
Solubilization in Digitonin 7. Solubilization Since callose and cellulose synthases
are membrane-bound, the first step in their purification must be solubilization.
10
The Proteomics Protocols Handbook
10. The extraction protocol described here represents a modification of a method
described by Fey et al. for fractionation of MDCK cells (4). Modifications include
the addition of a digitonin extraction step, the inclusion of EDTA in digitonin and ...
10 NEWS ITEMS WHICH INCLUDE THE TERM «DIGITONIN»
Find out what the national and international press are talking about and how the term
digitonin is used in the context of the following news items.
Mitochondrially targeted vitamin E succinate efficiently kills breast …
NativePAGE Novex Bis-Tris (4-16 % gradient) gels (Life Technologies) were used for electrophoresis of digitonin-solublised mitochondria. «BMC Blogs Network, May 15»
The Case Against Open Design Competitions
The Vietnam Veterans Memorial, Flickr user digitonin. Consider the design contest held by the Solomon R. Guggenheim Foundation for a new ... «Co.Design, Mar 15»
Flight Rebooking is Only Getting More Confusing in the US
Skift Global Forum. digitonin / Flickr. Crews work to de-ice a plane at Seattle-Tacoma International Airport. digitonin / Flickr ... «Skift, Oct 14»
Another Side to Hong Kong: Explore the Best Nature Destinations
While it may be best known for Disneyland and the city's airport, Lantau Island is home to some of Hong Kong's best scenery. Photo by digitonin. «The Epoch Times, Oct 14»
Racist, Anti-Asian Flier Rocks UCLA, USC Campuses
Tuesday, February 11, 2014 | 1 year ago. Racist, Anti-Asian Flier Rocks UCLA, USC Campuses. digitonin/Flickr. A racist flier was sent to Asian American student ... «LA Weekly, Feb 14»
Benefits of Using Scanning Monochromators in Fluorometric …
However, when detergents such as triton or digitonin are added to solubilize membranes, they can significantly lower the background because ... «News-Medical.net, Dec 13»
Autophagic failure promotes the exocytosis and intercellular transfer …
To observe the amount of membrane-bound LC3 in the tfLC3-overexpressing cells, the cells were permeabilized briefly with digitonin as ... «Nature.com, May 13»
Crystal structure and biochemical analyses reveal Beclin 1 as a …
After wash cells with PBS 3 times for ten min each, incubate for 10 min with PBS containing 0.1% saponin or 30 min with 100 μM digitonin on ... «Nature.com, Feb 12»
The Smell of Success: Researchers Embed Olfactory Receptors …
The researchers surmounted this problem by purifying the OR proteins from murine (mouse) cells and solubilizing them into digitonin micelles ... «Medgadget.com, Aug 11»
A carbon nanotube transistor that can smell
To fabricate their sensor, the researchers first purified ORs from cells and then solubilized them in two distinct nanoscale constructs: digitonin ... «Nanowerk LLC, Jul 11»
