10 ENGLISH BOOKS RELATING TO «PUROMYCIN»
Discover the use of
puromycin in the following bibliographical selection. Books relating to
puromycin and brief extracts from same to provide context of its use in English literature.
500mg/ml puromycin: Dissolve 10mg of puromycin in 20ml of deionized double-
distilled water. Sterilize by filtration through a 0.45-mm polysulfone filter. Store
aliquots at -20°C. Steps 1. Thaw primary human fibroblasts in complete medium
X.
2
Molecular Biology: Genes to Proteins
The antibiotic puromycin, which is produced by the gram-positive bacteria
Streptomyces alboniger, helped to solve the problem. Puromycin is remarkably
similar in structure to the tyrosyl- adenosine group at the 3'-end of Tyr-tRNA (
FIGURE ...
3
Biochemistry and Molecular Biology of Antimicrobial Drug Action
Peptidyl—puromycin then dissociates from the ribosome. Provided that the
peptidyl-tRNA is in the P site on the ribosome, its reaction with puromycin has no
other requirement than a normally functioning peptidyl transferase activity.
Puromycin ...
Trevor J. Franklin, George Alan Snow, 2006
4
Neurobiology of Learning and Memory
Would recent memory be destroyed by this treatment and lunger-term memory be
preserved? Shock was omitted in the retention trials 3 days after injection of
puromycin since ihere was, within the design of the test, no right or wrong
response.
Gordon L. Shaw, James L. McGaugh, 1990
5
The Antimicrobial Drugs
The release by puromycin of radioactive phenylalanyl-tRNA prebound to
ribosomes: with and without tetracycline. '4C-labeled phenylalanyl-tRNA was
bound to ribosomes with and without tetracycline. The phenylalanyl-tRNA was
present in ...
Eric Michael Scholar, William B. Pratt, 2000
6
Embryonic Stem Cells: Methods and Protocols
Methods and Protocols Kursad Turksen. Table 1 Selection Agents for Genetically
Modified ES Cells Drug concentration Marker gene Selection (μg/mL) Neomycin
phosphotransferase (neo) Positive G418 (300) Puromycin N-acetyl transferase ...
7
INTERNATIONAL REVIEW NEUROBIOLOGY
biotic, acetoxycycloheximide, evidently negates the effect of another, puromycin (
Flexner and Flexner, 1966). Other complications include the observation that
intracerebral injections of isotonic saline (Flexner and Flexner, 1967) , as well as
...
Carl Curt Pfeiffer, John Raymond Smythies, John R Smythies, MD, Frcp, 1975
8
Cancer Biology and Biosynthesis
Table 1: Effeets of Cycloheximide and Histidinol on the Synthesis of Peptidyl-3H-
puromycin. Cells were incubated for 10 min in the presence of 10" M H-
puromycin (1 mCi/mraol). Synthesis of peptidyl-3H-puromycin was determined as
...
9
Chemistry of Nucleosides and Nucleotides
Since puromycin is an analogue of the 3'-terminus of aa-tRNA, some of its
biological properties are very similar to those of 2'(3')-0-L-phenylalanyladenosine
(11; R = CH2-C6H5) (see Section 10). The main difference in structure between ...
10
Molecular Mechanisms of Protein Biosynthesis
Nascent peptides can be transferred to C-A-C-C-A(Phe) and similar aminoacyl-
oligonucleotides as to puromycin (Lessard and Pestka, 1972a,b). Also Rychlik et
al. (1969) have shown that aminoacyl-nucleosides are most inhibitory to protein ...
10 NEWS ITEMS WHICH INCLUDE THE TERM «PUROMYCIN»
Find out what the national and international press are talking about and how the term
puromycin is used in the context of the following news items.
Over 5000 base pairs were inserted into mammalian cells using a …
This assay ensures that only site-specific integration in proper orientation can give rise puromycin resistance. Protospacer adjacent motif (PAM) bases are in ... «Next Big Future, Jul 15»
Kruppel-like factor 4 signals through microRNA-206 to promote …
... CO, USA). The retroviral vector pBABEpuro-HA-KLF4 and viral transduction was previously described. Cells were selected using puromycin (1 μg/ml). «Nature.com, Jun 15»
CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis …
Alternatively, Hep-NTCP cells were selected with puromycin following transduction and subsequently infected with HBV-positive patient serum (Fig S8 right). «Nature.com, Jun 15»
MacroH2A1 downregulation enhances the stem-like properties of …
Subsequently, the cells were infected with virus particles and the puromycin-selected cells were pooled. MacroH2A1 open reading frame in the pIRES-FLAG ... «Nature.com, Jun 15»
A novel antibody–drug conjugate targeting SAIL for the treatment of …
Stably transfected populations were selected in 0.2 μg/ml puromycin for 7 days before the cytotoxicity assay. One representative experiment of two is shown. «Nature.com, May 15»
HEDGEHOG/GLI-E2F1 axis modulates iASPP expression and …
... treated with the CDK1 inhibitor JNJ-7706621 (500 nM, JNJ; Merck, Damstadt, Germany) for 72 h. Puromycin was used at 2 μg/ml to select for transduced cells. «Nature.com, May 15»
The Aurora A-HP1γ pathway regulates gene expression and mitosis …
HP1γ (Cbx3) knockdown was achieved by lentiviral shRNA (Santa Cruz Biotechnology, Inc), according to manufacturer's instructions with puromycin selection (2 ... «BMC Blogs Network, May 15»
The oncogenic role of the cochaperone Sgt1
MEF cells were infected with retroviral supernatant twice and selected in the presence of 2 μg/ml of puromycin (Sigma-Aldrich, St. Louis, MO, USA) for 4 days. «Nature.com, May 15»
Advertorial: Comparing RNAi and CRISPR Technology for Loss-of …
After three days of growth, cells were selected with puromycin then growth maintained with replating as necessary. Fifty million cells were harvested for each ... «Genetic Engineering & Biotechnology News, Apr 15»
Glomerular disease A “double hit” can injure podocytes
In the present study, GqQ>L mice were administered puromycin aminonucleoside (PAN), to determine whether this additional challenge to the podocyte could ... «Nature.com, Apr 15»
