CONJUGATION OF THE VERB TO TRANSFECT
PRESENT
Present
I transfect
you transfect
he/she/it transfects
we transfect
you transfect
they transfect
Present continuous
I am transfecting
you are transfecting
he/she/it is transfecting
we are transfecting
you are transfecting
they are transfecting
Present perfect
I have transfected
you have transfected
he/she/it has transfected
we have transfected
you have transfected
they have transfected
Present perfect continuous
I have been transfecting
you have been transfecting
he/she/it has been transfecting
we have been transfecting
you have been transfecting
they have been transfecting
Present tense is used to refer to circumstances that exist at the present time or over a period that includes the present time. The
present perfect refers to past events, although it can be considered to denote primarily the resulting present situation rather than the events themselves.
PAST
Past
I transfected
you transfected
he/she/it transfected
we transfected
you transfected
they transfected
Past continuous
I was transfecting
you were transfecting
he/she/it was transfecting
we were transfecting
you were transfecting
they were transfecting
Past perfect
I had transfected
you had transfected
he/she/it had transfected
we had transfected
you had transfected
they had transfected
Past perfect continuous
I had been transfecting
you had been transfecting
he/she/it had been transfecting
we had been transfecting
you had been transfecting
they had been transfecting
Past tense forms express circumstances existing at some time in the past,
FUTURE
Future
I will transfect
you will transfect
he/she/it will transfect
we will transfect
you will transfect
they will transfect
Future continuous
I will be transfecting
you will be transfecting
he/she/it will be transfecting
we will be transfecting
you will be transfecting
they will be transfecting
Future perfect
I will have transfected
you will have transfected
he/she/it will have transfected
we will have transfected
you will have transfected
they will have transfected
Future perfect continuous
I will have been transfecting
you will have been transfecting
he/she/it will have been transfecting
we will have been transfecting
you will have been transfecting
they will have been transfecting
The
future is used to express circumstances that will occur at a later time.
CONDITIONAL
Conditional
I would transfect
you would transfect
he/she/it would transfect
we would transfect
you would transfect
they would transfect
Conditional continuous
I would be transfecting
you would be transfecting
he/she/it would be transfecting
we would be transfecting
you would be transfecting
they would be transfecting
Conditional perfect
I would have transfect
you would have transfect
he/she/it would have transfect
we would have transfect
you would have transfect
they would have transfect
Conditional perfect continuous
I would have been transfecting
you would have been transfecting
he/she/it would have been transfecting
we would have been transfecting
you would have been transfecting
they would have been transfecting
Conditional or "future-in-the-past" tense refers to hypothetical or possible actions.
IMPERATIVE
Imperative
you transfect
we let´s transfect
you transfect
The
imperative is used to form commands or requests.
NONFINITE VERB FORMS
Past participle
transfected
Present Participle
transfecting
Infinitive shows the action beyond temporal perspective. The
present participle or gerund shows the action during the session. The
past participle shows the action after completion.
10 ENGLISH BOOKS RELATING TO «TRANSFECT»
Discover the use of
transfect in the following bibliographical selection. Books relating to
transfect and brief extracts from same to provide context of its use in English literature.
1
Attempts to
Transfect the Prion Protein in Human Cancer Cell ...
Transmissible spongiform encephalopathies (TSE) or prion diseases are a group of fatal neurodegenerative diseases that affects humans and animals.
Khor Heng Wei, Yeoh Bee Yoong, Toh Li Fang, 2011
2
RNA Interference Technology: From Basic Science to Drug ...
As discussed above, one advantage of adenoviral delivery is the ability to
efficiently infect non-dividing or hard-to-transfect cell types that may be difficult to
transfect by standard methods. In such cases, action of the shRNA may be
masked ...
Krishnarao Appasani, 2005
3
Advances in AIDS-Related Dementia Research and Treatment: ...
New research, 'Carbosilane dendrimers to transfect human astrocytes with small
interfering RNA targeting human immunodeficiency virus,' is the subject of a
report. According to recent research from Madrid, Spain, “HIV infection of the
CNS is ...
4
Frontiers in Medicinal Chemistry
Ability to transfect quiescent cells. Short duration of expression. Inflammatory host
reaction. Able to accommodate relatively large transgenes. Easy to produce in
high titers. Retroviruses Long-lasting gene expression. Low immunogenicity.
Atta-ur- Rahman, Allen B. Reitz, 2006
5
Dissecting Cell Fate Decisions in Human Embryonic Stem Cells
To test the ability of nucleofection to transfect hES cells and to optimize
conditions for high efficiency transfection, a 3.5 kb plasmid expressing GFP from
the CMV promoter (GFP vector) was transfected into H9 hES cells and the
percentage of ...
Kristi A. Hohenstein, 2009
6
DNA Conformation and Transcription
... 5 HM PO TFO 1 1 mer CT ODN, -TFO 52 IgH 3' a enhancer, Pax5 site, plasmid
& chromosomal 41 mer GT POTFO, 3'- amine, Pu3 EMSA/ in vivo DMS FP in vivo,
CH12.LX.A2 cells, luciferase, northern, (co)transfect TFO +1 60% ...
Ann Marie Craig Neurons are difficult to transfect because they are postmitotic
cells; thus, methods that allow entry of DNA into the cytoplasm are not sufficient.
In dividing cells, if one can get the DNA into the cytoplasm, it will enter the
nucleus ...
Gary Banker, Kimberly Goslin, 1998
8
Cellular and Molecular Methods in Neuroscience Research
OVERVIEW We describe two simple, quick, and reproducible methods to
transfect primary hippocampal neurons. The first method consists of a modified
calcium phosphate (CP) transfection. We have developed this method to analyze
...
Adalberto Merighi, Giorgio Carmignoto, 2002
9
Nucleic Acid Transfection
4.3.2 MVLBisG2 Efficiently Transfects MEFs, a Hard-to-Transfect Cell Line
Another important discovery was made when comparing MVLBisG2 and DOTAP
in a number of different cell lines. As shown in Fig. 12, complexes of MVLBisG2 ...
Wolfgang Bielke, Christoph Erbacher, 2010
10
Understanding Retroviral Replication: Roles of Nucleocapsid ...
B. B. MLV Gag-Pol Transfect MLV Gag-Pol Transfect GFFP provirus GFFP
provirus + + SV-A-MLV-env SV-A-MLV-env or or MLV Gag-Pol Transfect MLV
Gag-Pol Transfect NC+ RT - NC+ RT - + + GFFP provirus SV-A-MLV-env + GFFP
provirus ...
10 NEWS ITEMS WHICH INCLUDE THE TERM «TRANSFECT»
Find out what the national and international press are talking about and how the term
transfect is used in the context of the following news items.
Transfection Reagents and Equipment Market by Method, & by …
NEW YORK, July 8, 2015 /PRNewswire/ -- Over the years, the transfection reagents and equipment market has witnessed various technological advancements ... «PR Newswire, Jul 15»
Single Walled Carbon Nanotubes Can Transfect PBMCs Following …
The ability of SWCNT to enable in vivo transfection of PBMCs would be a significant accomplishment for this field which may lead to novel therapeutic ... «AZoNano.com, Jul 15»
Transfection Reagents and Equipment Market Worth $957.9 Million …
According to a new market research report "Transfection Reagents and Equipment Market by Method (Biochemical (Calcium Phosphate, Lipofection, ... «PR Newswire, Jul 15»
Nanosheet transfection: effective transfer of naked DNA on silica glass
We demonstrate that naked DNA can be transferred into difficult-to-transfect cells (for example, stem cells) by plating cells on silica glass with the upright ... «Nature.com, Jun 15»
Top Trends in Biopharmaceutical Manufacturing: 2015
“Avian lines (e.g., duck embryo quail sarcoma and chick embryo fibroblasts) have been reported to transfect well, have promoters that work with mammalian ... «Pharmaceutical Technology Magazine, Jun 15»
GEN Roundup on Transfection Techniques
Transfection is the definitive method for deliberately introducing DNA or RNA into cells, most often for gene therapy purposes. There are several technical ... «Genetic Engineering & Biotechnology News, May 15»
GE Healthcare Webinar Will Highlight RNAi Success in Difficult-to …
In contrast, siRNA application in difficult-to-transfect cell types (such as primary or suspension cells) proves to be extremely labor-intensive and technically ... «PR Web, Apr 15»
Highly Efficient Targeted mRNA Delivery
Offering high transfection efficiency and expression levels across a broad range of cell types, including stem cells and primary cells, mRNA-In™ is a robust and ... «The Scientist, Mar 15»
Transfection Transitions to Bioproduction
Transfection, the delivery of nucleic acids into cells, is the core technique for enhancing or inhibiting gene expression, or for initiating the expression of nonnative ... «Genetic Engineering & Biotechnology News, Dec 14»
AMSBIO announces DNA-In™ Neuro Transfection Reagent
AMSBIO announces DNA-In™ Neuro- a new generation transfection reagent developed specifically for maximum nucleic acid delivery into neurons, typically ... «News-Medical.net, Dec 14»
