MOTS EN ANGLAIS EN RAPPORT AVEC «SPHEROPLAST»
spheroplast
buffer
yeast
protocol
transformation
protoplast
cell
from
which
wall
been
almost
completely
removed
action
penicillin
name
stems
fact
that
after
microbe
digested
membrane
tension
causes
acquire
characteristic
spheroplast
merriam
webster
bacterium
modified
enzymatic
there
partial
loss
increased
osmotic
sensitivity
medical
bacterial
fungal
results
removal
rigid
forms
bound
with
define
gram
negative
altered
partly
missing
resulting
origin
sphero
plast
oxford
dictionaries
british
world
meaning
pronunciation
example
sentences
reference
content
saccharomyces
cerevisiae
during
formation
prospheroplast
could
lysed
like
retained
covered
preparations
were
shadow
cast
electron
microscopy
autolytic
mechanism
bacillus
cereus
escherichia
coli
bacteriol
strain
life
technologies
contains
qualified
reagents
preparation
have
optimized
pichia
pastoris
openwetware
materials
plates
10 LIVRES EN ANGLAIS EN RAPPORT AVEC «SPHEROPLAST»
Découvrez l'usage de
spheroplast dans la sélection bibliographique suivante. Des livres en rapport avec
spheroplast et de courts extraits de ceux-ci pour replacer dans son contexte son utilisation littéraire.
1
METHODS IN CELL BIOLOGY,VOLUME 32: VESICULAR TRANSPORT, PART ...
conversion is monitored by following the decrease in absorbance at OD600
when 10 pl of spheroplast suspension is diluted into 1 ml of water. The
conversion is complete when the OD600 is 5-10% of the initial OD,,,,0. Under a
phase-contrast ...
Alan Michael Tartakoff, 1989
2
METHODS IN CELL BIOLOGY,VOLUME 12: YEAST CELLS: YEAST CELLS
The amount of enzyme necessary to produce spheroplasts can vary with the
yeast strain used and its phase of growth when harvested. Cells grown to the
midlog phase are usually significantly easier to spheroplast than stationary-
phase cells.
3
Methods in Practical Laboratory Bacteriology
This method, based on that originally described for £ coli by Witholt et al.,27
involves the conversion of the cells into spheroplasts, followed by lysis to yield
outer- and inner- membrane protein vesicles. This spheroplast fractionation
method ...
4
Developmental Biology Protocols
1) including spheroplast fusion of yeast containing a YAC with totipotent mouse
embryonic stem (ES) cells, lipofection of purified YAC DNA into ES cells, and
microinjection of purified YAC DNA into single-cell mouse embryos. In addition,
the ...
Rocky S. Tuan, Cecilia W. Lo, 2000
3.4.4 Spheroplast fusion Spheroplast fusion provides another way of overcoming
the mating/ sporulation barrier found in industrial yeasts but, unlike rare mating, it
is a direct asexual technique. It can be used to produce both hybrids and ...
Fergus Priest, Iain Campbell, 2003
6
Advanced Techniques in Chromosome Research
The following steps describe a modified spheroplast transformation protocol that
provides a high-transformation efficiency, at 1-2 x 10* colonies/ p.g of intact
vector [76]. The efficiency is much lower for ligation products; a good ligation
reaction ...
7
Functional Genetics of Industrial Yeasts
2.3.4 Protoplast / spheroplast fusion Protoplast or spheroplast fusions offer a
means of genetic transfer where interspecific breeding is not possible, or where
exchanges between different species and genera are desired (Russell and
Stewart ...
Johannes H. de Winde, 2003
Wash the spheroplast preparation by centrifugation at 1500g for 10 min once in
10 mL of 1 M sorbitol and once in 10 mL of CaS. Spheroplasts are very fragile.
Therefore, decant supernatants carefully, and resuspend gently by tapping the
side ...
David R. Higgins, James M. Cregg, 1998
Visualization of the VSV G protein in the spheroplast plasma membrane. (A-C)
VSV was fused with the spheroplasts at pH 4 at 37°C. The fixed spheroplasts
were subjected to immu- noelectron microscopy using anti-G protein antibody ...
10
Studies on Dynamics and Function of Bacteriorhodopsin from ...
6.4.2 Spheroplast preparation For each transformation a 2 mL cell suspension
was harvested in a 2 mL Eppendorf tube at 2000 x g, room temperature, for 3 min
. The cell pellet was gently suspended in 0.2 mL of SPH. Subsequently, 20 pL ...
3 ACTUALITÉS CONTENANT LE TERME «SPHEROPLAST»
Découvrez de quoi on parle dans les médias nationaux et internationaux et comment le terme
spheroplast est employé dans le contexte des actualités suivantes.
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole …
Spheroplast formation was carried out according to published protocols. Unless indicated otherwise, all steps were performed at 4 °C. Cells (30 mg) were ... «Nature.com, oct 11»
Plants feel the force
Not that spheroplasts are that big. “We're doing most of our studies in Xenopus oocytes (frog eggs), whose diameters are 150 times bigger than those of ... «Washington University in St. Louis News, oct 11»
Get Rid of What Makes Lyme Disease Tick
It can assume spirochete, spheroplast or L shaped, and cyst forms. Besides changing form, leading to mistaken identity and mis-diagnosis, it can lodge deep ... «Natural News.com, juil 10»
