CHE SIGNIFICA CDNA IN INGLESE
DNA complementare
Nella genetica, il DNA complementare è il DNA sintetizzato da un template di messaggistica RNA in una reazione catalizzata dalla transcriptasi inversa degli enzimi e dalla polimerasi di DNA. Il cDNA è spesso utilizzato per clonare geni eucarioti nei prokaryotes. Quando gli scienziati vogliono esprimere una proteina specifica in una cellula che normalmente non esprime tale proteina, trasferirà il cDNA che codifica la proteina alla cellula ricevente. Il cDNA è anche prodotto naturalmente da retrovirus e poi integrato nel genoma dell'ospite dove crea un provirio. Il termine cDNA è anche usato, tipicamente in un contesto bioinformatico, per riferirsi a una sequenza di trascrizione mRNA, espressa come basi di DNA piuttosto che basi RNA.
definizione di cDNA nel dizionario inglese
La definizione di cDNA nel dizionario è DNA complementare; una forma di DNA sintetizzata artificialmente da un modello di RNA messaggero e utilizzata nell'ingegneria genetica per produrre cloni genici.
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10 LIBRI IN INGLESE ASSOCIATI CON «CDNA»
Scopri l'uso di
cDNA nella seguente selezione bibliografica. Libri associati con
cDNA e piccoli estratti per contestualizzare il loro uso nella letteratura.
1
Generation of
CDNA Libraries: Methods and Protocols
Expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences.
2
Positional Cloning by Exon Trapping and
CDNA Selection
This comprehensive guide details the exon-trapping and cDNA selection processes step by step--from isolating genomic templates and nuclear splicing, to verifying generated clones and sequenced data, to analyzing exon libraries and cDNA ...
3
Generation of Full-length
CDNA Clone and Functional Analysis ...
Papain-like leader proteases are found in diverse families of human, animal, plant, and fungal positive-strand RNA viruses.
4
cDNA preparation and characterization
This volume presents an update of a number of approaches relevant to the areas referred to above.
Sherman M. Weissman,
1999
5
Microarray Technology and Cancer Gene Profiling
Single-Strand cDNA Synthesis A critical step in RNA or cDNA amplification is the
generation of double-stranded cDNA (ds-cDNA) templates. First-strand cDNAs
are reverse transcribed from mRNA using oligo-dT or random primers. In order to
...
6
Characterization of a Membrane Protein Encoding
CDNA ( ...
The genetic control of desiccation tolerance was not fully characterized.
7
Construction of Recombinant Rabbit Uterine Smooth Muscle ...
DISCUSSION Synthesis of Full Length Open Reading Frame of SmMLCK cDNA
Gallagher etal., (1991a) characterized rabbit uterine MLCK gene using lambda gt
cDNA libraries, and multiple piecing of restriction digested products together.
Satishkumar Noonepalle,
2008
8
Handbook of Molecular and Cellular Methods in Biology and ...
Construction of Fractional cDNA Libraries Using Xenopus Oocytes as an
Expression System 175 Reagents Needed 180 V. cDNA Cloning and Analysis by
the Polymerase Chain Reaction 180 A. Selection of Oligonucleotides 181 B.
General ...
Leland J. Cseke, Peter B. Kaufman, Gopi K. Podila,
2003
Introduction Although Thermus aquaticus (Taq) and Thermus thermophilus (Tth)
DNA polymerases have the ability to reverse transcribe RNA to complementary
DNA (cDNA) and subsequently amplify the target cDNA, they are not usually the
...
Bing-Yuan Chen, Harry W. Janes,
2002
10
Principles and Technical Aspects of PCR Amplification
Starting poly A 5 material SMART oligo dG PCR adaptor poly A 5 RACE dC RT
RT First-strand cDNA synthesis Second-strand cDNA synthesis cDNA dC dT
Oligo (dT) primer with added sequence PCR amplification or primer extension ...
Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays,
2008
10 NOTIZIE DOVE SI INCLUDE IL TERMINE «CDNA»
Vedi di che si parla nei media nazionali e internazionali e come viene utilizzato il termine ino
cDNA nel contesto delle seguenti notizie.
Transcriptome analysis of the biofilm formed by methicillin …
The cDNA libraries of nine samples (Supplementary Table S1 online) were pooled and subjected to high-throughout sequencing. «Nature.com, lug 15»
MAOA expression predicts vulnerability for alcohol use
Standard curves for each gene (rhβ-actin and rhMAOA), were prepared using serial dilutions of cDNA (from 25 ng to 0.125 ng). Real-time PCR ... «Nature.com, lug 15»
Genetic screening reveals a link between Wnt signaling and …
For either cDNA overexpression or siRNA knockdowns, we seeded 104 HEK293 or A23 cells into 96-well plates. After 24 h, 50 ng of ZNRF3 ... «Nature.com, lug 15»
Decreased LRIG1 in fulvestrant-treated luminal breast cancer cells …
Total RNA was quantified and 2 μg RNA was used to synthesize cDNA (High Capacity cDNA Reverse Transcription kit, Applied Biosystems ... «Nature.com, lug 15»
Differential miRNA expressions in peripheral blood mononuclear …
cDNA was diluted 100 × and assayed in 10 μl PCRs according to the protocol for miRCURY LNA™ Universal RT miRNA PCR; each miRNA ... «Nature.com, lug 15»
Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation
cDNA was synthesized using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA, USA). The SYBR Green DNA Master mix (Applied ... «Nature.com, lug 15»
Ecophysiology of an uncultivated lineage of Aigarchaeota from an …
Double-stranded complementary DNA (cDNA) was synthesized at the Department of Energy-Joint Genome Institute from the OS total RNA ... «Nature.com, lug 15»
Cytomegalovirus infection induces a stem cell phenotype in human …
cDNA was synthesized from equal amounts of RNA with the SuperScript III First-Strand Synthesis System (Invitrogen, Life Technologies) and ... «Nature.com, lug 15»
Heat-Denatured Lysozyme Inactivates Murine Norovirus as a …
The resulting cDNA was subjected to a Real-time PCR, using the SYBR Premix Ex Taq II kit (Takara Bio). The PCR was performed with 2.0 μL ... «Nature.com, lug 15»
DNA Gene Chip Market Segment Forecasts up to 2020, Research …
Based on type, DNA gene chip market can be segmented as oDNA (oligonucleotide DNA) and cDNA (complementary DNA). The former is a ... «CSO Australia, lug 15»