«COCULTIVATION»に関連する英語の本
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cocultivationの使いかたを見つけましょう。
cocultivationに関する本と英語文献で使われた文脈を提供するための簡単な抜粋文。
1
Transgenic Plants and Crops
Results from Transformation Experiments After cocultivation, epicotyl explants
were placed on selective medium with 25 mg I of kanamycin for 4 weeks.
Subsequently, healthy calli were subcultured to fresh selective medium
containing 100 mg ...
George C. Khachatourians, Y. H. Hui, Ralph Scorza, 2002
2
Transgenic Plants: Engineering and Utilization
Methods for cocultivation of A. tumefaciens with leaf disc tissue have been
described in detail (Horsch et al., 1985; Rogers et al., 1986). Cocultivation with
other excised plant tissues follows the same basic procedure. A brief description
of the ...
It is very important to wash the Agrobacterium culture with MSS medium prior to
cocultivation to ensure that the antibiotic (used as an Agrobacterium strain-
specific selection) is sufficiently removed and does not affect the growth of the ...
Julio Salinas, Jose J. Sánchez-Serrano, 2006
Preculture ofexplants Preculture and cocultivation are the important steps in
Agrobacterium-mediated transformation. The process of preincubation makes the
explant tissue competent enough to withstand the bacterial infection and other ...
5
PROG NUCLEIC ACID RES&MOLECULAR BIO
(106) compared infection rates by cocultivation, using either N2 packaged by
PA12 cells containing amphotropic helper virus or N2 packaged by the helper-
free amphotropic packaging cell line, PA317. The percentage of G418-resistant ...
Waldo E. Cohn, Kivie Moldave, 1990
6
Agrobacterium Protocols Volume 2
The amount of glucose is lower in the solid cocultivation IM medium than the
standard IM, because too much sugar leads to overgrowth of the yeast and lower
T-DNA transfer. 5. Several different haploid and diploid yeast strains (including ...
7
Handbook of Industrial Mycology
a Phosphate-free. b To induce the vir genes during the cocultivation period,
acetosyringone (3′- 5′-dimethoxy-4-hydroxyacetophe-none; Aldrich catalog
number D-13440–6) is added to the GI agar at a final concentration of 200 μM,
from a ...
8
Nuclease Methods and Protocols
4. Decant the bleach, and rinse five times with autoclaved deionized water. 3.3.2.
Preparing Explants Sterile leaves are cut into pieces 0.5–0.7 cm on a side using
a #11 scalpel. Discard large veins. Arrange the explants on cocultivation plates.
Catherine H. Schein, 2001
9
Plant Protoplasts and Genetic Engineering V
Many of the calli used in the transformation experiments remained on induction
medium for at least 6 months prior to bombardment or cocultivation. About 500
mg fresh weight (fr. wt.) of embryogenic tissues was plated on fresh induction ...
10
Handbook of Biodegradable Polymers: Isolation, Synthesis, ...
Especially the cocultivation with fibroblasts from the oral mucosa induced the
regeneration of a morphologically and functionally regular tracheal epithelium.
This was comparable to the regeneration of epithelium in vitro after cocultivation
with ...
Andreas Lendlein, Adam Sisson, 2013
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Three-Dimensional Cultivation of Cells
... specific temperature, adequate perfusion, cocultivation with separated feeder cells, change of certain parameters during cultivation, etc.). «Genetic Engineering News, 2月 09»