«ORCINOL»に関連する英語の本
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1
Lab Manual in Biochemistry: Immunology and Biotechnology
AIM: To estimate RNA quantitatively using orcinol reagent. PRINCIPLE This
method relies on the conversion of pentose sugar, ribose, present in RNA into
furfural in the presence of hot acid. Furfural then reacts with orcinol in the
presence of ...
Orcinol Method In RNA, there is ribosyl moiety. Ribose is measured by orcinol
method which will be indicative of the RNA concentration. As in case of
diphenylamine method, here too a standard series of RNA is run for comparison.
The orcinol ...
Anil Kumar, Neha Garg, 2005
3
An Introduction to Practical Biotechnology
ESTIMATION OF RNA BY ORCINOL METHOD Principle This is a general
reaction for pentose and depends on the formation of furfural when pentose is
heated with concentration l-lCL orcinol reacts in presence of furfusal in presence
of ferric ...
4
METHODS IN MICROBIOLOGY
This is followed by 0-2 ml of a freshly prepared 6% solution of orcinol in 95%
ethanol. The mixture is heated in a boiling water bath for 20 min and its optical
density read at 665 nm. It should be noted that other sugars give colours with this
...
Douglas W. Ribbons, J. R. Norris, 1972
5
Essentials of Biotechnology
LEARNING OBJECTIVE After performing this practical you will able to: • Explain
significance of quantitatie estimation of RNA • Explain principle of Orcinol method
• Estimate the content of RNA INTRODUCTION RNA is single stranded, long, ...
Ulhas K. Patil, Kalyani Muskan, 2009
6
Methods of Biochemical Analysis
acid or orcinol, and under optimal conditions is complete after 30 min. heating at
100°C. However, increasing the concentration of reagents also deepens the
color of the blank determination and this sets a limit to the choice of reactant ...
7
Practical Biochemistry for Medical Students
THE ESTIMATION OF RNA BY MEANS OF THE ORCINOL REACTION This is a
general reaction for pentoses and depends on the formation of furfural when the
pentose is heated with concentrated hydrochloric acid. Orcinol reacts with the ...
8
The Chemistry and Biology of Sialic Acids and Related Substances
vided the material under examination is free from pyrroles or does not contain in
addition to sialic acid other substances convertible to pyrrole in acid medium.
Bial's orcinol reagent and its resorcinol variation (Svennerholm) are sensitive ...
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online.
Lambert M Surhone, Mariam T Tennoe, Susan F Henssonow, 2011
10
Ana Techniqs in Biotechnology
Goutam Bhowmik. Aim Estimation of nucleic acid. To estimate the RNA isolated
from a given sample. OR Spectrophotometric analysis of RNA/orcinol
determination of RNA. Place 3.0 ml of each standard and 3.0 ml of. Biology %
Experiment i ...
