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B. Digestion of Nuclei with DNAase I DNAase I (bovine pancreatic
deoxyribonuclease) is an endonuclease that has been widely used to analyse
the chromatin structure of many eukaryotic organisms. This enzyme degrades
double-stranded ...
2
General Cytochemical Methods
due to tissue DNAase and tissue phosphatases. Moreover, it must be determined
whether the procedure reveals the actual sites ofenzyme activity in the tissue, as
the precipitation technique presents several risks in this regard (Danielli, 1953, ...
These changes were first detected by the effects of digestion with very low
concentrations of the enzyme DNAase I. When chromatin is digested with
DNAase I, the first effect is the introduction of breaks in the duplex at specific
hypersensitive ...
4
Replication and Recombination of Genetic Material
The initial lag in appearance of transformants is most easily explained by the
existence of two steps leading to irreversible DNAase insensitive absorption of
donor DNA, as pointed out by Stocker et al (1953) and others. (See Bodmer,
1967, for ...
William James Peacock, Richard Donald Brock, 1968
5
Understanding DNA: The Molecule and How It Works
Certain other enzymes and chemicals, for example DNAase I, can cut the DNA in
practically any location, with only a mild specificity for the base sequence. How
might such a generalized DNA-cutting activity be useful? Suppose we have ...
Chris R. Calladine, Horace Drew, 1997
6
ADVANCES IN VIRUS RESEARCH
They found that when the pepsindigested virus was then treated with DNAase
there was, surprisingly at first sight, very little further change in the appearance of
the virus. However, when virus that had received pepsin treatment followed by ...
Kenneth Manley Smith, 1962
7
Electrotransformation of Bacteria
3 Post Pulse Events DNAase I effect Post pulse DNAase treatment of the plasmid/
cells mixture affects the yield of transformation. For bacteria (Eynard, et al, 1992,
Kimoto and Taketo, 1997) addition of DNAase I just after the pulse provoked a ...
Natalie Eynard, Justin Teissie, 2000
The original articles on enzymatic debridement for loculated parapneumonic
effusions used Varidase, which consists of a fibrinolytic (streptokinase) and a
DNAase (streptodornase). It is unclear how much the DNAase contributed to the
...
9
Between Nucleus and Cytoplasm
Most low ionic strength methods rely on disruption of the chromatin by DNAase
treatment, followed by repeated washing of the envelopes. An exception is the
heparin procedure of Bornens and Courvalin (1978) mentioned earlier. By far the
...
10
Genetic Engineering Techniques: Recent Developments
Isolation of Specific Genes in the Form of Transcriptionally Active Chromatin
Recent studies have shown that transcriptionally active chromatin is more
sensitive tQWard DNAase I than the inactive chromatin. The hypersensitive site
has been ...
WIADOMOŚCI, KTÓRE ZAWIERAJĄ SŁOWO «DNAASE»
Sprawdź, o czym dyskutuje się w prasie krajowej i zagranicznej oraz jak jest stosowane słowo
DNAase w wiadomościach.
Previously healthy 2-year-old presents with sore throat and limp
Post-streptococcal reactive arthritis must be differentiated from acute rheumatic fever, both of which may be associated with elevation of anti-DNAase B and ... «Healio, Cze 13»
Exploring the Genotype of the Tea Plant
Then the DNA is treated with anti-DNAase enzyme to prevent other enzymes from breaking up the DNA, and protease enzymes are added to ... «AllAfrica.com, Lut 13»
Junk DNA, Junky PR
The EP consortium labs systematically catalogued such things as DNAase I hypersensitive and methylated sites, transcription factor (TF) ... «Scientific American, Wrz 12»
New method to fight bacterial infections linked to contact lenses
So, they used the enzyme DNAase together with negatively charged poly aspartic acid to break down the chemical bonds of these elements ... «TopNews, Sty 11»
