KSIĄŻKI POWIĄZANE ZE SŁOWEM «LIGASE»
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1
Nucleic Acid Amplification Technologies: Application to ...
This book is designed for all biomedical scientists interested in the application of molecular biology to clinical diagnosis."--BOOK JACKET.Title Summary field provided by Blackwell North America, Inc. All Rights Reserved
Helen H. Lee, Stephen A. Morse, Ørjan Olsvik, 1997
2
Enzymology Primer for Recombinant DNA Technology
RNA LIGASE: T4 RNA LIGASE [Polyribonucleotide synthase (ATP), EC 6.5.1.3]
RNA ligases catalyze the 3' — > 5' phosphodiester bond formation of RNA
molecules with concomitant hydrolysis of ATP to AMP and PP; . Initially detected
as an ...
3
Base Excision Repair of DNA Damage
replication, at least during early embryogen- esis.46 Studies on DNA replication
and DNA repair in cell lines established from these DNA ligase I mutant embryos
should prove informative. DNA LIGASE II PHYSICAL AND BIOCHEMICAL ...
This was readily demonstrated in the first step, the formation of ligase-adenylate,
but was technically difficult to prove in the next two steps, the formation and
consumption of DNA-adenylate. An interesting kinetic feature is the activation of
E.
Arthur Kornberg, Tania A. Baker, 2005
5
RNA-Protein Interactions : A Practical Approach: A Practical ...
ligase' reflected this ability (29, 30). Like T4 RNA ligase, T4 DNA ligase joins the
3' OH of a 5' acceptor polynucleotide to a 5' terminal monophosphate of a 3'
donor polynucleotide in the presence of ATP. However, unlike T4 RNA ligase, ...
Christopher W.J. Smith, 1998
6
A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis
Isolation, Analysis, and Synthesis Paul A. Krieg. merase I, and RNase H, and E.
coli DNA ligase. The conditions and enzyme concentrations for second-strand
cDNA synthesis have been optimized to produce high yields of double-stranded
...
7
Molecular Biology: A Project Approach
Ligase, an enzyme that uses ATP to form bonds, is used in recombinant DNA
cloning to join restriction endonuclease fragments that have annealed. The
ligase commonly used is T4 DNA ligase, which was first isolated from E. coJi that
were ...
8
Recombinant DNA Principles and Methodologies
The ligase has a relatively low turnover number of 25 sealing events per minute
per ligase molecule. The polyionic polymer PEG 8000 greatly enhances the rate
of phosphodiester bond formation by ligase, presumably by increasing the local ...
9
Proteomics of Spermatogenesis
multi-cellular eukaryotes contain more than one species of DNA ligase. DNA
ligase I is required for DNA replication involved in DNA repair. Pol-[5 and DNA
ligase-l seem to be components of a multi-protein complex that performs base ...
10
Experimental Techniques in Bacterial Genetics
co/i and S. typhimurium have an NAD dependent DNA ligase that is essential for
repairing nicks generated during DNA replication and recombination in vivo . The
DNA ligase commonly u sed for DNA cloning is made by phage T4. In contrast ...
WIADOMOŚCI, KTÓRE ZAWIERAJĄ SŁOWO «LIGASE»
Sprawdź, o czym dyskutuje się w prasie krajowej i zagranicznej oraz jak jest stosowane słowo
ligase w wiadomościach.
Spectrum and frequencies of BRCA1/2 mutations in Bulgarian high …
... in the RING domain and abolished the ubiquitin ligase activity of BRCA1 protein as well as its participation in the homologous recombination ... «BMC Blogs Network, Lip 15»
Genome-wide gene–gene interaction analysis for next-generation …
Article | PubMed |; Emanuele MJ, Elia AE, Xu Q et al: Global identification of modular cullin-RING ligase substrates. Cell 2011; 147: 459–474. «Nature.com, Lip 15»
Digestion of Nucleic Acids Starts in the Stomach
3c). As shown in Fig. 3c, if a fragment has a 3′-phosphate, its 3′ end will not be ligated to another synthesized oligo-DNA by T4 DNA ligase, ... «Nature.com, Lip 15»
Surveying the repair of ancient DNA from bones via high-throughput …
Both methods use endonucleases and glycosylases along with a DNA polymerase-ligase combination. PreCR Repair Mix increases the number of molecules ... «BioTechniques.com, Lip 15»
WesternU Researchers Unveil New Mechanism in the Regulation of …
This study shows that a type of SK, the synaptic SK2 channels, are regulated by the E3 ubiquitin ligase UBE3A, whose deficiency results in ... «Benzinga, Lip 15»
Not All Dengue Viruses Have Have Same Potential For Disease …
This subgenomic RNA then binds a host protein, TRIM25, which is a E3 ubiquitin ligase that polyubiquitylates RIG-I to amplify and sustain ... «Medical Research News and Interviews on MedicalResearch.com, Lip 15»
The proinflammatory role of HECTD2 in innate immunity and …
We discovered a ubiquitin E3 ligase, HECTD2, which ubiquitinated and mediated the degradation of PIAS1, thus increasing inflammation in an experimental ... «Science Magazine, Lip 15»
A nonsense variant in HERC1 is associated with intellectual …
HERC1 is an ubiquitin ligase that interacts with tuberous sclerosis complex 2, an upstream negative regulator of the mTOR pathway. Our data ... «Nature.com, Lip 15»
Promotion of Endoplasmic Reticulum-Associated Degradation of …
Depletion of particular ERAD-associated isomerases, lectins, and translocon components, including ERAD E3 ubiquitin ligase HRD1, ... «Journal of Virology, Lip 15»
Hybrid vigour: Infertility rescue
The male sterility thus induced is rescued in hybrids because TBPm3 is tagged with an additional protein fragment used by the ubiquitin ligase, ... «Nature.com, Lip 15»
