KSIĄŻKI POWIĄZANE ZE SŁOWEM «POLYSOME»
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1
Translation Initiation: Extract Systems and Molecular ...
The ultimate test of in vivo translation deficiency is to measure the abundance of
polysome by sucrose gradient-velocity sedimentation, in which extracts prepared
from growing yeast are layered onto a sucrose density gradient and resolved by ...
2
Yeast Gene Analysis: Yeast Gene Analysis
I Polysome analysis of translation initiation A defect in protein synthesis may be
suspected when gene disruption or mutation results in a phenotype such as
antibiotic sensitivity. In such cases polysome analysis can be used to probe the ...
3
Methods in Plant Cell Biology
polyribosome (“polysome”) complexes. Further, not all polysome-associated
mRNAs are translated in vivo; some are arrested. Translational arrest has been
observed following acute changes in the environment, such as the onset of
hypoxia ...
4
RNA Methodologies: A Laboratory Guide for Isolation and ...
Purification of Polysome-Engaged mRNA Although mRNA is an excellent
parameter of gene expression, thorough assessment of this aspect of the cellular
biochemistry must be multifaceted. Standard RNA isolation techniques, even
when ...
Robert E. Farrell, Jr.,
2010
5
mTOR Pathway and mTOR Inhibitors in Cancer Therapy
However, pull-downs would, in comparison to polysome preparations, result in
reduced resolution. During polysome preparations the researcher has the choice
to stratify in different ways, so that, e.g., fractions with mRNA bound to greater ...
Vitaly A. Polunovsky, Peter J. Houghton,
2010
6
• mRNA Life Cycle in Brain Function and Malfunction:
FIGURE 2 | Schematic of genome-wide methods to study polysome-associated
mRNAs in vitro and in vivo. (A)With polysome profiling, cytoplasmic lysates from
cells are layered onto a sucrose gradient and undergo centrifugation to separate
...
Jernej Ule, Peter Scheiffele, Michael A. Kiebler,
2014
7
Combinatorial Chemistry
Procedure for Polysome Binding Assay Ligate an individual sequence or a pool
of amplified DNA sequences into the NheliKpnl sites of pLM193. Electroporate E.
coli strain XLl- Blue and isolate individual ampicillin-resistant colonies as ...
Penicillinase polysome size Attempts were made to measure the size of the
penicillinase polysome on sucrose gradients. That the enzyme activity peaks in
the polysome region mainly represented nascent chains, rather than free enzyme
...
9
Aromatic Amino Acids in the Brain
On the basis of pharmacological evidence, we suspect that both dopamine and
5hydroxytryptamine influence polysome aggregation by acting on
pharmacologically-specific receptors. Methysergide and cyproheptadine block
receptors for ...
CIBA Foundation Symposium,
2009
10
Living in a Seasonal World: Thermoregulatory and Metabolic ...
Significant insight for mechanism is provided by polysome analyses. In polysome
analyses, the number of ribosomes that associate with a given mRNA is related
to the amount of translation. In actively translating systems, as many as 20–30 ...
Thomas Ruf, Claudia Bieber, Walter Arnold,
2012
WIADOMOŚCI, KTÓRE ZAWIERAJĄ SŁOWO «POLYSOME»
Sprawdź, o czym dyskutuje się w prasie krajowej i zagranicznej oraz jak jest stosowane słowo
polysome w wiadomościach.
RNA-binding protein HuR sequesters microRNA-21 to prevent …
Polysome analysis showed that PDCD4 mRNA was mostly present in the non-translating lighter fractions of the MCF7-miR-21 cells compared ... «Nature.com, Lip 15»
Lost in translation? Not when it comes to control of gene expression …
... three sophisticated analytical techniques: global polysome profiling to measure active translation for thousands of mRNAs; ribosome footprint ... «Phys.Org, Maj 14»
Pretreatment Genetic Test Predicts Recurrence in Prostate Cancer
The effect of hypoxia on the translation of more than 220 genes was measured using polysome-translation assays. These tests demonstrated ... «OncLive, Kwi 14»
A technique to increase protein yield in a rabbit reticulocyte lysate …
Recombinant NS1RK/AA enhances protein synthesis in RRL and facilitates polysome formation in RRL. (Click to enlarge). To further determine the effect of ... «BioTechniques.com, Sty 14»
Scientists Discover Chemical Modification in Human Malaria …
Le Roch and colleagues published two other papers about their findings in November—“Polysome profiling reveals translational control of ... «UC Riverside, Gru 13»