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METHODS IN CELL BIOLOGY,VOLUME 32: VESICULAR TRANSPORT, PART ...
conversion is monitored by following the decrease in absorbance at OD600
when 10 pl of spheroplast suspension is diluted into 1 ml of water. The
conversion is complete when the OD600 is 5-10% of the initial OD,,,,0. Under a
phase-contrast ...
Alan Michael Tartakoff, 1989
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METHODS IN CELL BIOLOGY,VOLUME 12: YEAST CELLS: YEAST CELLS
The amount of enzyme necessary to produce spheroplasts can vary with the
yeast strain used and its phase of growth when harvested. Cells grown to the
midlog phase are usually significantly easier to spheroplast than stationary-
phase cells.
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Methods in Practical Laboratory Bacteriology
This method, based on that originally described for £ coli by Witholt et al.,27
involves the conversion of the cells into spheroplasts, followed by lysis to yield
outer- and inner- membrane protein vesicles. This spheroplast fractionation
method ...
4
Developmental Biology Protocols
1) including spheroplast fusion of yeast containing a YAC with totipotent mouse
embryonic stem (ES) cells, lipofection of purified YAC DNA into ES cells, and
microinjection of purified YAC DNA into single-cell mouse embryos. In addition,
the ...
Rocky S. Tuan, Cecilia W. Lo, 2000
3.4.4 Spheroplast fusion Spheroplast fusion provides another way of overcoming
the mating/ sporulation barrier found in industrial yeasts but, unlike rare mating, it
is a direct asexual technique. It can be used to produce both hybrids and ...
Fergus Priest, Iain Campbell, 2003
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Advanced Techniques in Chromosome Research
The following steps describe a modified spheroplast transformation protocol that
provides a high-transformation efficiency, at 1-2 x 10* colonies/ p.g of intact
vector [76]. The efficiency is much lower for ligation products; a good ligation
reaction ...
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Functional Genetics of Industrial Yeasts
2.3.4 Protoplast / spheroplast fusion Protoplast or spheroplast fusions offer a
means of genetic transfer where interspecific breeding is not possible, or where
exchanges between different species and genera are desired (Russell and
Stewart ...
Johannes H. de Winde, 2003
Wash the spheroplast preparation by centrifugation at 1500g for 10 min once in
10 mL of 1 M sorbitol and once in 10 mL of CaS. Spheroplasts are very fragile.
Therefore, decant supernatants carefully, and resuspend gently by tapping the
side ...
David R. Higgins, James M. Cregg, 1998
Visualization of the VSV G protein in the spheroplast plasma membrane. (A-C)
VSV was fused with the spheroplasts at pH 4 at 37°C. The fixed spheroplasts
were subjected to immu- noelectron microscopy using anti-G protein antibody ...
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Studies on Dynamics and Function of Bacteriorhodopsin from ...
6.4.2 Spheroplast preparation For each transformation a 2 mL cell suspension
was harvested in a 2 mL Eppendorf tube at 2000 x g, room temperature, for 3 min
. The cell pellet was gently suspended in 0.2 mL of SPH. Subsequently, 20 pL ...
WIADOMOŚCI, KTÓRE ZAWIERAJĄ SŁOWO «SPHEROPLAST»
Sprawdź, o czym dyskutuje się w prasie krajowej i zagranicznej oraz jak jest stosowane słowo
spheroplast w wiadomościach.
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole …
Spheroplast formation was carried out according to published protocols. Unless indicated otherwise, all steps were performed at 4 °C. Cells (30 mg) were ... «Nature.com, Paz 11»
Plants feel the force
Not that spheroplasts are that big. “We're doing most of our studies in Xenopus oocytes (frog eggs), whose diameters are 150 times bigger than those of ... «Washington University in St. Louis News, Paz 11»
Get Rid of What Makes Lyme Disease Tick
It can assume spirochete, spheroplast or L shaped, and cyst forms. Besides changing form, leading to mistaken identity and mis-diagnosis, it can lodge deep ... «Natural News.com, Lip 10»
