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카스테레오에서 찬송가가 흘러나오고 있다. 멀리서 떠오르는 태양이 눈부시다. 너무 평온한 가슴을 만져 본다. 집이 보인다. 지난 3일 동안 이런 상상이 머리를 떠나지 않 고 있다. 연구실의 16,000rpm으로 돌아가는 센트리 퓨지 앞에서도 세파덱스 칼럼 ...
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Column Handbook for Size Exclusion Chromatography - 39페이지
A Sephadex Sephadex, the first commercially available gel—filtration media, is formed from cross-linking dextran with epichlorohydrin and is available as a dry powder or in various prepacked column types for desalting. The various Sephadex ...
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Liquid Column Chromatography: A Survey of Modern ... - 785페이지
In fact, this decreases to a certain extent the differences in capacity between ion exchangers with a matrix of the Sephadex (1-25 and G-50 type. The ratios of the capacities for haemoglobin and ion exchangers derived from the polydextran ...
K. Macek, Z. Deyl, J. Janák,
2011
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A Guide to Protein Isolation - 105페이지
Because it consists of a large volume of water immobilised by a relatively small amount of gel, Sephadex G-200 is relatively soft and is best used with ascending elution. Figure 73. The cross-liking of dextran chains to form Sephadex gel.
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Protein Analysis and Purification: Benchtop Techniques - 142페이지
The medium chosen should totally exclude the target protein. A suitable quantity of Sephadex G-25 or Biogel P-30 is packed in a column and equilibrated with the desired new buffer (see Chapter 10 for details). The sample volume should not ...
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Proteins: Biochemistry and Biotechnology - 121페이지
Sephadex G-25 or Bio Gel P2, have pore sizes that exclude all proteins from entering the gel matrix. Such gels may be used to separate proteins from other molecules which are orders of magnitude smaller, and are often used to remove low ...
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Immunofluorescence in Clinical Immunology: A Primer and Atlas
The antigen, as an example, thyroglobulin, is linked to a carrier such as Sephadex G25, which is itself labelled with a fluorescent dye. The spherules are then incubated with the cells to be investigated. Cells with antigen-specific receptors bind ...
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Practice of Thin Layer Chromatography - 34페이지
3.9 SEPHADEX Sephadex is the trade name for a group of modified dextran gels for gel filtration that are produced by Pharmacia Fine Chemicals, Inc. Sephadex gels are hydrophilic, neutral stationary phases that may be used in thin layers.
Joseph C. Touchstone,
1992
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Encyclopedia of Surface and Colloid Science - 4권 - 3228페이지
Since i/q is also inversely proportional to the square root of W, the conformational change of the Sephadex particle would be caused by a movement of dextran chains.1651 The conformational change of the Sephadex particle is discussed ...
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Chemical and Functional Properties of Food Proteins - 89페이지
Commercially available gel filtration media are mainly marketed as Sephadex G (cross-linked dextrans), Bio-Gel P (poly- acrylamide), Bio-Gel A (agarose chains), Sepharose 2B (agarose gels), and Superdex (covalently binding dextran to ...
Zdzislaw E. Sikorski,
2001