10 УКРАЇНСЬКА КНИЖКИ ПОВ'ЯЗАНІ ІЗ «ДЕНАТУРАНТ»
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1
Molecular Tools for Screening Biodiversity: Plants and Animals
lene-bis-acrylamide). HAZARD!! for deionization see Note 2. 5. Denaturant stock solution 1 (0%): 6.5% acrylamide in 1x TAE (81 ml 40% acrylamide, and 25 ml 20x TAE in 500 ml H.O). 6. Denaturant stock solution 2 (80%): 6.5% acrylamide, ...
A. Karp, D.S. Ingram, Peter G. Isaac, 2012
2
Biothermodynamics - Частина 2 - Сторінка 344
For both unfolding and refolding kinetics, it is necessary to ''jump'' to different final denaturant concentrations. The basic rationale is because determining the dependence of the observed rates on the denaturant concentration allows ...
Michael L. Johnson, Jo M. Holt, Gary K. Ackers, 2009
3
Introduction to Protein Science: Architecture, Function, ... - Сторінка 359
no denaturant dilute denaturant more denaturant GN GD G N GD GN GD Figure 8.6 Dependence of free energy of native and denatured states on denaturant concentration. The energy of interaction of peptides with denaturant is greater than ...
4
Technologies for Detection of DNA Damage and Mutations
In this case the electrophoretic migration is perpendicular to the denaturant gradient and the sample is applied along the entire width of the gel. In this way each DNA molecule will travel along a path of constant denaturant concentration at a ...
5
Protein Engineering Handbook - Сторінка 104
By contrast, the values of [Denaturant]50% or Tm tend to be more robust to the vagaries of curve fitting10) and, for this reason, we use these parameters to determine accurate values of ΔGD-N or ΔΔGD-N [70]. Where a given protein chemically ...
Stefan Lutz, Uwe Theo Bornscheuer, 2012
6
Biophysical Tools for Biologists: In Vitro Techniques - Сторінка 305
A more serious error in DG H2O may arise if DG varies nonlinearly with denaturant. Although values within the transition appear to be linear, any well-behaved function is approximately linear over a narrow range, and the transition is indeed ...
John J. Correia, H. William Detrich, III, 2011
7
Protein Stability and Folding: A Collection of ... - Сторінка 7
b) Linear extrapolation: In K°unf= In Kunft madenaturam) (11a) Or Arg-Ang”-man" (11b) c) Transfer model: Aunt G -: A°unf(; + 8 X. Ili ög", (12) Case (a) – Denaturant binding model: Among the equations introduced in I-69A is eq. (10) agrees ...
8
G Proteins - Сторінка 384
Other modifications to this technique include (1) melting of the duplexes in solution using step-wise increase in denaturant concentration prior to standard polyacrylamide electrophoresis, (2) constant denaturant gel electrophoresis (CDGE) ...
9
Methods in Protein Structure and Stability Analysis: ... - Сторінка 63
In chemical denaturation, additives are titrated into the sample while monitoring the folded state of the protein using some structural probe. Three popular denaturants are (in order of increasing denaturing power) urea, guanidine hydrochloride ...
Vladimir N. Uversky, Evgeniĭ Anatolʹevich Permi︠a︡kov, 2007
10
Methods to Study Litter Decomposition: A Practical Guide
Clean all glass plates with ethanol and assemble them with 1-mm spacers according to the manufacturer's instructions for parallel DGGE. 3. In a 50-ml plastic tube prepare 16 ml of a low-denaturant solution containing 20% denaturing gradient ...
Manuel A.S. Graça, Felix Bärlocher, Mark O. Gessner, 2007
