与 «MICROCOCCAL»相关的英语书籍
在以下的参考文献中发现
micrococcal的用法。与
micrococcal相关的书籍以及同一来源的简短摘要提供其在 英语文献中的使用情境。
1A), and an appropriate concentration of micrococcal nuclease is selected for
digestion of the residual nuclear suspension, which is kept on ice after the first
titration step. In the experiment displayed in Fig. 1A, we selected a concentration
of ...
Paul M. Wassarman, Alan P. Wolffe, 1999
2
RNA Polymerase and Associated Factors
Micrococcal Nuclease Digestion Assay Micrococcal nuclease preferentially
digests DNA in internucleosomal (linker) regions of the chromatin. Thus, partial
digestion with the nuclease should reveal periodic spacing of assembled
nucleosomes ...
Sankar Adhya, Susan Garges, 2003
21 Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome
Positioning Zhengjian Zhang and Joseph C. Reese Summary Chromatin
structure and nucleosome positioning play a crucial role in gene expression
regulation.
4
Nucleic Acid Research: Future Development
plain the rather low frequency of cleavage of micrococcal nuclease at this site (
indicated by the small arrow in Fig. 5 of Samal et al., 1981, and by the broken line
in Fig. 3 below). This enhanced micrococcal nuclease cleavage is the only ...
5
Viral Gene Techniques: Viral Gene Techniques
The distribution of DNA topoisomers and the nucleosomal repeat (as determined
by micrococcal nuclease digestion; see below) are identical for salt-treated and
native minichromosomes. However, protein analysis reveals clear differences ...
6
Chlamydial Infections: Proceedings of the Sixth ...
Micrococcal nuclease (1 yg/ml ) was used to digest unprotected DNA. Reactions
were run in 50 mM Tris, pH 8.0, 37°C, for one hour. A similar reaction was used
for DNAase I, (1 yg/ml ) . Pronase and proteinase K. Some samples were treated
...
7
Molecular Biology of the Cell, Fifth Edition: The Problems Book
When you digest rat liver nuclei briefly with micrococcal nuclease, extract the
DNA, and run it on an agarose gel, it forms a ladder of broad bands spaced at
about 200nucleotide intervals. If you use the enzyme DNase I instead, there is a
much ...
John Wilson, Tim Hunt, 2012
8
PROG NUCLEIC ACID RES&MOLECULAR BIO
C. Micrococcal Nuclease Analysis Confirms That Active Genes Reside within an
Altered Chromatin Subunit It has often been stated that, while DNase I exhibits a
preference for "active" DNA sequences in chromatin, micrococcal nuclease does
...
9
Eucaryotic Gene Regulation
The presence of a unique and discrete DNA fragment pattern generated by
cleavage with DNase I, and to a lesser extent by micrococcal nuclease, at
particular chromosomal loci suggests that the cleavage sites are position—
specific with ...
In this section we describe a method to digest nuclei with micrococcal nuclease
that has been successful in generating nucleosome ladders from maize and
Arabidopsis chromatin. (1) Resuspend the nuclei preparation in an adequate
volume ...
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This Week in PNAS
In their proof-of-principle application of this approach, the study's authors compared MPE-seq with micrococcal nuclease (MNase) sequencing ... «GenomeWeb, 六月 15»
A streamlined ribosome profiling protocol for the characterization of …
Lysate is then treated with micrococcal nuclease in a buffer that maintains ribosome integrity without sacrificing high nuclease activity. Monosomes are then ... «BioTechniques.com, 六月 15»
Inorganic Arsenic-induced cellular transformation is coupled with …
To this end, we used several methods to test for chromatin structural changes - bulk nucleosome repeat length (NRL), micrococcal (MNase) ... «BMC Blogs Network, 三月 15»
10 Tips for Successful ChIP Result Using Chromatrap® Technology
Both enzymatic (micrococcal nuclease digestion) shearing and sonication (mechanical shearing utilizing ultrasonics) are effective shearing ... «News-Medical.net, 十二月 14»
Setting the stage for chromosome breakage
Chromatin structure influences DSB formation in many organisms, and so the authors made use of data generated previously by micrococcal ... «Fred Hutchinson Cancer Research Center News, 九月 14»
Chromatin Immunoprecipitation: 10 Technical Tips for Success
Following cell lysis, chromatin is sheared by either sonication or enzymatic digestion with micrococcal nuclease (MNase) to generate ~500 bp ... «SelectScience.net, 九月 14»
GSBS student receives HHMI International Student Research …
Instead of using restriction enzymes, I simply fragment chromatin by micrococcal nuclease and enable single nucleosome resolution for ... «News from the University of Massachusetts, 八月 14»
A rapid and efficient method to purify proteins at replication forks …
The niPOND protocol suggests the use of micrococcal nuclease to digest chromatin, followed by overnight extraction in buffer containing 0.1% Triton-X. Using ... «BioTechniques.com, 十月 13»
4 Steps to Get Your ChIP Experiment Working
When doing N-ChIP, enzymatic digestion with micrococcal nuclease should be able to generate single monosomes (~175bp). Keep in mind that micrococcal ... «Genetic Engineering News, 七月 13»
Our necessary but 'dirty' cell phones
Bacteria grown on cell phones include staphylococcus, streptococcus and micrococcal organisms in amounts large enough to potentially make ... «Inquirer.net, 六月 12»
