与 «PARAFORMALDEHYDE»相关的英语书籍
在以下的参考文献中发现
paraformaldehyde的用法。与
paraformaldehyde相关的书籍以及同一来源的简短摘要提供其在 英语文献中的使用情境。
1
Immunocytochemistry: Theory and Practice
In fact, when combined with cryostat sectioning, this procedure has made
possible the localization of the overwhelming majority of antigens sought Freshly
prepared paraformaldehyde solutions are, however, much weaker fixatives than
...
2
Practical Flow Cytometry
Paraformaldehyde, glutaraldehyde, acrolein, and osmium tetroxide at various
concentrations were tested as fixatives; staining of Thy-1, T-2090, Lyt-l, Lyt-Z, and
Th-B antigens on murine and of B1, B2, Bz-microglobulin, HLA—DR, CALLA, ...
3
Techniques in Quantification and Localization of Gene Expression
8 9 10 FIXATION vs RNA QUALITY FIXATION vs RNA YIELD FRESH
PARAFORMALDEHYDE ETHANOL MOCK FRESH PARAFORMALDEHVDE
ETHANOL in this chapter will be divided into fixation of cells and fixation of
tissues, since ...
The most commonly used fixation method for leukocytes which are to be
analyzed by flow cytometry is that reported by Lanier and Warner (1981) for
murine lymphocytes. This procedure ultilizes 1% paraformaldehyde in saline for
postfixation.
Zbigniew Darzynkiewicz, J. Paul Robinson, Harry A. Crissman, 1994
23-'' This action was precipitated by a number of lawsuits, including an out-of-
court settlement for $1,000,000 to a woman horribly disfigured after
paraformaldehyde paste was misused (R. Uchin, personal communication,
September 22, 1992) ...
John Ide Ingle, Leif K. Bakland, 2002
6
METHODS IN CELL BIOLOGY,VOLUME 31: VESICULAR TRANSPORT, PART ...
STOCK SoLvTIoNs 40% Paraformaldehyde (Merck) in H2O 80 mM K-PIPES (pH
6.5), 5 mM EGTA, 2 mM MgCl2 100 mM NaB407 (pH 11.0) + 0.02% saponin 2.
Preparation Of The Stock Solutions The preparation of the paraformaldehyde ...
Alan Michael Tartakoff, 1989
Which would be more viscous? Explain your reasoning. Polyacetals. Another
oxygen-containing polymer is paraformaldehyde, a polyacetal formed by the
addition polymerization of formaldehyde, a reaction that takes place as an
aqueous ...
Marye Anne Fox, James K. Whitesell, 2004
8
Immunocytochemistry: A Practical Guide for Biomedical Research
There are two different sources of formaldehyde, formalin and paraformaldehyde.
Formalin is commercially produced by oxidation of methanol and contains 37%
formaldehyde and impurities including 14% methanol, small amounts of formic ...
9
Purification of Laboratory Chemicals
Add KOH solution (1 mole KOH: 100 moles HCHO) to ~37% by weight aqueous
formaldehyde solution (formalin), or evaporate to dryness, to give
paraformaldehyde polymer which, after washing with water, is dried in a vacuum
desiccator over ...
W.L.F. Armarego, Christina Chai, 2009
10
Methods in Avian Embryology
Fix embryos. The choice of fixative and fixation time largely depend on the nature
of the experiment, what antibodies are used, and the size of the embryo. For most
experiments, embryos may be fixed in 4% paraformaldehyde overnight at 4°C.
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Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated …
At 48 h post-transfection, cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and blocked for 1 h at ... «Nature.com, 七月 15»
Fetal hematopoietic stem cells express MFG-E8 during mouse …
Tissue specimens were fixed with 4% paraformaldehyde (Duksan, Ansan, Gyeonggi, Korea) at room temperature for at least 12 h. «Nature.com, 七月 15»
Circadian and ultradian rhythms of clock gene expression in the …
... mice were transcardially perfused with physiological saline solution followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB) under ... «Nature.com, 七月 15»
Targeting hedgehog signaling reduces self-renewal in embryonal …
For immunofluorescence, cells were fixed with 4% paraformaldehyde and incubated over night at 4 °C with primary antibodies. All the ... «Nature.com, 七月 15»
Nrf2 is a potential prognostic marker and promotes proliferation and …
The cells were washed with cold PBS, fixed in 4 % paraformaldehyde, permeabilized with 0.3 % Triton X-100, blocked with 5 % bovine serum ... «BMC Blogs Network, 七月 15»
RNA-binding protein HuR sequesters microRNA-21 to prevent …
Cells were fixed with 4% paraformaldehyde and treated with 1:200 diluted mouse monoclonal anti-HuR antibody (Santa Cruz Biotechnology) ... «Nature.com, 七月 15»
KLF5 promotes breast cancer proliferation, migration and invasion in …
The cells migrating to the lower side of the membrane were fixed in 4% paraformaldehyde for 20 min at room temperature, stained with crystal ... «Nature.com, 七月 15»
STAT3-dependent reactive astrogliosis in the spinal dorsal horn …
... followed by 50 ml ice-cold 4% paraformaldehyde/PBS (PFA). Cervical and lumbar segments of the dorsal root ganglion (DRG) and spinal cords were removed ... «Nature.com, 七月 15»
Analysis of the TGFβ-induced program in primary airway epithelial …
... 4 % paraformaldehyde in PBS. Afterwards, the fixed cells were stained with Alexa Fluor® 568 phalloidin for cytoplasmic distribution of F-actin ... «BMC Blogs Network, 七月 15»
Peripheral blood cells from children with RASopathies show …
Cells were then fixed with paraformaldehyde and permeabilized with ice-cold methanol according to published methods and then incubated ... «Nature.com, 七月 15»
