与 «SUPERNATANT»相关的英语书籍
在以下的参考文献中发现
supernatant的用法。与
supernatant相关的书籍以及同一来源的简短摘要提供其在 英语文献中的使用情境。
The other lighter organelles remain floating in the clear supernatant fluid above
the sediment. Differential centrifugation, the technique by which the organelles
inside cells can be isolated, g is the unit of gravitational force: spinning at 600g ...
Michael Roberts, Michael Jonathan Reiss, Grace Monger, 2000
2
Handbook of Molecular and Cellular Methods in Biology and ...
Extract the supernatant with 1 volume of chloroform:isoamyl alcohol (24:1).
Centrifuge as in step 7. Transfer the supernatant to a fresh tube. 12. To the
supernatant, add 0.1 volume of 3M sodium acetate (pH 5.2) and 2-4 volumes of
chilled ...
Leland J. Cseke, Ara Kirakosyan, Peter B. Kaufman, 2011
3
Embryonic Stem Cells: Methods and Protocols
If desired, test supernatant in an ELISA (see Subheading 3.7.). g. Purify antibody
(Subheading 3.10.). 5. For harvesting antibodies from periplasmic space: a.
Innoculate 50 mL 2X TY media with 2% glucose, 100 μg/mL ampicillin. b. When
the ...
4
Wastewater Sludge Processing
Odor problems in an anaerobic digestion system are associated primarily with
supernatant liquid and with storage and dewatering of digested sludge.
Supernatant is not drawn off from single-stage digesters. In two-stage systems
also, ...
Izrail S. Turovskiy, P. K. Mathai, 2006
18 Viral Genome Methods titer of retroviral producers can be increased by
harvesting supernatant after incubation at 32°C, as opposed to 37°C, but
supernatant can be harvested at either temperature.49 The medium may be
changed as ...
6
Molecular Biology: Genes to Proteins
The crude cell extract is centrifuged at about 600 g for five minutes to remove
intact cells and nuclei, and then the post-nuclear supernatant is centrifuged at
about 15,000 g for one hour to remove mitochondria, lysosomes, and
peroxisomes.
7
Development of Plant-Based Medicines: Conservation, Efficacy ...
To quickly recover cells from the cryo-stress, one efficacious strategy is to use the
supernatant from regular suspension culture. Supernatant can be added into
solid or liquid medium. The application of supernatant to the post-thaw recovery ...
8
Mitochondrial Function, Part B: Mitochondrial Protein ...
The supernatant, containing the mitochondria, is transferred to new 1.7-ml tube
and placed on ice while additional mitochondria trapped in the pellet are
harvested two more times to yield the maximum amount of mitochondria as
follows.
9
Applications of Chimeric Genes and Hybrid Proteins, Part A: ...
tional protein can be dependent on temperature. Diabodies usually give higher
functional yields at lower temperatures (e.g., 25). 4. Incubate (a) for 12 hr (30) to
24 hr (22) to harvest antibody fragments secreted into the culture supernatant or ...
10
Diarrhoeal Diseases Research
RESULTS In Figure 1, the mean fluid accumulation did not differ significantly
between suckling Wistar rats given culture supernatant containing ST- producing
ETEC alone and those given the same culture supernatant mixed with berberine
at ...
包含词语«SUPERNATANT»的新条目
找出国内和国际出版社所讨论的内容，以及词语
supernatant在以下新条目的上下文中是如何使用的。
T cells A killer cytokine
In addition, supernatant from cultures of human TH17 cells but not of TH0 cells (which do not express IL-26) efficiently killed extracellular bacteria, an effect that ... «Nature.com, 七月 15»
Computational Prediction and Validation of BAHD1 as a Novel …
(B) Detection of IL-6, IL-8 and MCP-1 secretion in cell culture supernatant by ELISA, the siBAHD1 group is reported as the fold increase compared with the ... «Nature.com, 七月 15»
Overexpression of Hsp70 confers Cytoprotection during Gliadin …
... the non-cytoskeletal supernatant fraction. Recovery from gliadin exposure was associated with induction and cytoskeletal redistribution of Hsp70. The in-vitro ... «Nature.com, 六月 15»
Optima X Series Ultracentrifuges and SW 32 TI Rotor for Lentiviral …
In order to eliminate any cell debris and loose cells, the viral supernatant is collected from transfected packaging cells and passed through a sterile 0.45µm filter. «News-Medical.net, 五月 15»
The Goldilocks of heterogeneous catalysis
Leaching of the metal catalyst from a support into solution is however a central problem in heterogeneous catalysis. Metals lost to the supernatant can be costly, ... «Royal Society of Chemistry, 四月 15»
A single protocol for extraction of gDNA from bacteria and yeast
The supernatant was removed, and 100 L of an optimized suspension solution (see Supplementary Material) containing 0.1 mM EDTA, 50 mM Tris-HCl, pH 8.0, ... «BioTechniques.com, 三月 15»
RNAPro•SAL: A device for rapid and standardized collection of …
Saliva supernatant (SS) containing freely soluble nucleic acids and proteins as well as exosome compar tmentalized RNA and proteins, along with a reduced ... «BioTechniques.com, 二月 15»
Technique enables improved sensing of toxic mercury compounds …
... followed by centrifugation and removal of the supernatant, which contains the free BSA (see image). When re-dispersed in buffer solution, the precipitate forms ... «Phys.Org, 九月 14»
What's in the Mount Polley Mine tailings pond?
Environmental Protection Division staff reviewed the tailings supernatant (i.e., the liquid in the tailings pond) data sent by Mount Polley Mining Corporation on ... «CKNW News Talk 980, 八月 14»
Fresenius Kabi Introduces Lovo Cell Processing System
Lovo, which is designed for laboratories, is an automated “cell-washing” system that removes supernatant from target cells and stores them in viable media. «Business Wire, 四月 14»
