CO OZNACZA SŁOWO COMPLEMENTARY DNA
Komplementarny DNA
W genetyce komplementarne DNA jest syntetyzowane DNA z szablonu RNA komunikatora w reakcji katalizowanej enzymatyczną odwrotną transkryptazą i polimerazą DNA. cDNA jest często używany do klonowania genów eukariotycznych w prokariotach. Kiedy naukowcy chcą wyrazić konkretne białko w komórce, która normalnie nie wyraża tego białka, przekażą cDNA kodujące białko do komórki biorcy. cDNA jest również produkowany naturalnie przez retrowirusy, a następnie zintegrowany z genomem gospodarza, gdzie tworzy prowirusowy. Określenie cDNA jest również używane, zazwyczaj w kontekście bioinformatycznym, do odniesienia do sekwencji transkryptu mRNA, wyrażonej jako podstawy DNA, a nie podstaw RNA.
Definicja słowa complementary DNA w słowniku
Definicja komplementarnego DNA w słowniku jest formą DNA sztucznie zsyntetyzowaną z matrycowego matrycy RNA i wykorzystywaną w inżynierii genetycznej do wytwarzania cDNA genów klonów.
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1
Gene Cloning and
DNA Analysis: An Introduction
8.3.3 mRNA can be cloned as complementary DNA Messenger RNA cannot itself
be ligated into a cloning vector. However, mRNA can be converted into DNA by
complementary DNA (cDNA) synthesis. The key to this method is the enzyme ...
2
Clinical Gynecologic Endocrinology and Infertility
The RNA is the template for amplification by first converting it into complementary
DNA. Polymerase chain reaction is used to detect microbes, providing results
within hours even in the presence of antimicrobial drugs. This method can detect
...
Marc A. Fritz, Leon Speroff,
2012
3
Recombinant
DNA Methodology II
Complementary DNA fractionation by subtractive hybridization, 308—309
immobilization on solid supports, 45 insertion into A expression vector. 309—
310, 312 library construction bacteriophage A expression vectors, 312—313
directional ...
4
Artificial
DNA: Methods and Applications
Hybridization experiments showed that PNA oligomers containing either of these
monomers form stable complexes with complementary DNA and RNA. When
hybridizing a thymine 14-mer comprised of the monomeric unit depicted in entry
30 ...
Yury E. Khudyakov, Howard A. Fields,
2002
5
Understanding Bioinformatics
... complementary sequence to AAG is not TTC but CTT (see Figure 1.3A). (A) 3'
sugar-phosphate backbone H H c .*>• hydrogen bond. The Structure of DNA and
RNA Two complementary DNA strands interact base pairing to form a double
helix.
Marketa J. Zvelebil, Jeremy O. Baum,
2008
6
DNA Technology: The Awesome Skill
They researched the possibility of using a DNA strand to recognize a
complementary DNA strand amid a mixture of other DNA strands, much as a key
might seek out a lock. Eventually, they succeeded and brought forth the
significant factor in ...
7
Academic Press Dictionary of Science and Technology
... complementary constant-current logic Electronics, a computer circuit design
that produces extremely fast switching speeds of 3 nanoseconds. complementary
deoxyribonucleic acid see complementary DNA. complementary DNA Genetics.
Christopher G. Morris,
1992
8
25th Southern Biomedical Engineering Conference 2009; 15 - ...
Judging by this result, it can be concluded that hybridization did not take place in
the region with non-complementary DNA. In Figure 4, it can also be seen that the
angles at which tunneling occurs increases in the region with complementary ...
Anthony McGoron, Chen-Zhong Li, Wei-Chiang Lin,
2009
9
Genes and
DNA: A Beginner's Guide to Genetics and Its ...
The specific bands are differentiated from the background DNA smear because
they can bind to the “complementary DNA” probe specific to a region ofthe β-
hemoglobin gene. The complementary DNA can be labeled with a dye for easy ...
Charlotte K. Omoto, Paul F. Lurquin,
2013
10
Enzymology Primer for Recombinant
DNA Technology
Length and nucleotide composition of DNA substrates (a) Minimum length of
substrates. Oligonucleotides as short as 6 or 7 (but not 4) bases in length can be
joined if annealed to long complementary DNA strands (6). Both E. coli and T4
DNA ...
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complementary DNA w wiadomościach.
OTUB1 inhibits the ubiquitination and degradation of FOXM1 in …
Complementary DNA generated by Superscript III reverse transcriptase and oligo-dT primers (Invitrogen, Paisley, UK) was analysed by ... «Nature.com, Lip 15»
Ecophysiology of an uncultivated lineage of Aigarchaeota from an …
Double-stranded complementary DNA (cDNA) was synthesized at the Department of Energy-Joint Genome Institute from the OS total RNA ... «Nature.com, Lip 15»
DNA Gene Chip Market Segment Forecasts up to 2020, Research …
Based on type, DNA gene chip market can be segmented as oDNA (oligonucleotide DNA) and cDNA (complementary DNA). The former is a ... «CSO Australia, Lip 15»
miR-16 rescues F508del-CFTR function in native cystic fibrosis …
Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 1989; 245: 1066–1073. | Article | PubMed ... «Nature.com, Lip 15»
Integrin α1β1 expression is controlled by c-MYC in colorectal cancer …
Complementary DNA was amplified using primers specific for ITGA1: forward 5′-CATCAGGTGGGGATGGTAAG-3′ and reverse ... «Nature.com, Cze 15»
Non-syndromic hearing loss caused by the dominant cis mutation …
The coding region of human Cx26 complementary DNA was subcloned into the pEGFP-N1 mammalian expressible plasmid using BamHI and ... «Nature.com, Cze 15»
Sygnis enters distribution agreement with Scandinavian Biotop for …
... two versions: SunScript RT RNaseH+ is optimized for the synthesis of complementary DNA (cDNA) as well as for use in reverse transcriptase ... «pharmabiz.com, Cze 15»
Eminent Virologist Dies
In 1996, he coauthored the first study demonstrating the production of an infectious RNA virus using complementary DNA. “It proved very ... «The Scientist, Cze 15»
Strand-specific RNA sequencing in Plasmodium falciparum malaria …
... complementary DNA (cDNA) using PCR and divergent primer pairs. We designed divergent ARP_circRNA primer pairs, as is depicted in Fig. «BMC Blogs Network, Cze 15»
Real-time probe illuminates snapper stocks
Senior supervising scientist Dr Mike Snow says they developed a snapper-specific probe which seeks out its complementary DNA sequence ... «Science Network Western Australia, Cze 15»