与 «LUCIFERASE»相关的英语书籍
在以下的参考文献中发现
luciferase的用法。与
luciferase相关的书籍以及同一来源的简短摘要提供其在 英语文献中的使用情境。
1
Bioluminescence and Chemiluminescence
THERMOINACTIVATION AND REACTIVATION OF FIREFLY LUCIFERASE N. N.
UGAROVA, E. I. DEMENTIEVA AND I. A. LUNDOVSKIKH Chemistry Department,
Moscow State University, 119899, Moscow, Russia E-mail: ...
2
Testing for Genetic Manipulation in Plants
The luciferase gene (luc) from the North American firefly Photinus pyralis has
been used as a reporter gene in a variety of eukaryotic (Ow et al. 1986; Millar et
al. 1992; Kost et al. 1995) and prokaryotic (Wood and DeLuca 1987) organisms.
John Flex Jackson, Hans F. Linskens, Ross B. Inman, 2002
3
RNA Turnover in Eukaryotes: Analysis of Specialized and ...
Always cotransfect the luciferase reporter gene with a gene that encodes an
internal standard (such as b-galactosidase) and lacks miREs in order to allow
data to be normalized. When using DNA transfection to examine downregulation
by ...
Lynne E. Maquat, Megerditch Kiledjian, 2011
4
Encyclopedia of Imaging
Luciferase gene Bioluminescence. Figure 1 Biological principle of
bioluminescent imaging of luciferase expression, (a) Firefly luciferase converts
the substrate luciferin into oxyluciferin and light. Tissue-specific transcription
factors bind to ...
to monitor continuous ATP synthesis in mammalian cells that takes advantage of
the luciferase–luciferin system. Firefly luciferase is widely used as a reporter for
gene expression to study promoter regulation in mammalian cells, but its ...
Liza A. Pon, Eric A. Schon, 2011
firefly luciferase activity and have revealed the following153-154: 1. Anesthetics
inhibit firefly luciferase activity at concentrations very similar to those required to
produce clinical anesthesia. 2. The potency of anesthetics as inhibitors of firefly ...
7
Signaling Through Cell Adhesion Molecules
Luciferase trans-activator reporter system Cell transfection — A day prior to
transfection, in a 6-well or 35 mm tissue culture plates, 3 to 4 x 105 cells/well are
seeded in 2 ml of complete growth medium (DMEM/ 10% fetal calf serum ...
8
Embryonic Stem Cell Protocols
To correct for differences in transfection efficiencies and to normalize the GFP
fluorescence intensity, measure luciferase activity from the same lysate using the
luciferase assay kit from Promega according to the manufacturer's instructions. 1.
9
Physical Methods for Microorganisms Detection
Firefly luciferase kinetics. The symbols have the following meanings: Eact, active
luciferase dimer; Box, oxidized luciferase; M, luciferase monomer; Einact, inactive
(denatured) luciferase; Lu, D-luciferin; Lox, oxyluciferin; E*.Lox energetically ...
10
Bioengineering in Cell and Tissue Research
Picture of A Firefly Beetle (identified luciferase: Firefly luciferase), B Gaussia
princeps (identified luciferase: Gaussia luciferase; GLuc), C Renilla reniformis (
identified luciferase: Renilla luciferase) and D Metridia longa (identified
luciferase: ...
Gerhard M. Artmann, Shu Chien, 2008
包含词语«LUCIFERASE»的新条目
找出国内和国际出版社所讨论的内容，以及词语
luciferase在以下新条目的上下文中是如何使用的。
Oncogene - KLF5 promotes breast cancer proliferation, migration …
The MCF10A cells were transfected with luciferase (Luc) siRNA and KLF5 siRNA for 48 h. The MCF7 cells were infected with green fluorescent ... «Nature.com, 七月 15»
miR-32 functions as a tumor suppressor and directly targets SOX9 in …
Furthermore, SOX9 was confirmed as a direct target of miR-32, using a luciferase reporter assay. Conclusion: These findings indicate that ... «Dove Medical Press, 七月 15»
Oncogene - RNA-binding protein HuR sequesters microRNA-21 to …
LPS treatment or miR-21 overexpression did not affect the RNA levels of the luciferase reporter gene constructs (Supplementary Figure 9). «Nature.com, 七月 15»
Sasser: What's to blame for the decrease in fireflies?
The light, he said, is created by a protein called luciferase. When it comes in contact with oxygen and energy, it splits a molecule called luciferin ... «Dallas Morning News, 七月 15»
Lynn Youngblood: Lightning bugs put on a backyard show
The taillight contains two rare chemicals, a heat resistant substrate called luciferin, is the source of light, and an enzyme luciferase is the trigger. «Blue Springs Examiner, 七月 15»
Downregulation of miR203 induces overexpression of PIK3CA and …
Luciferase activity was examined by using 3'-untranslated region luciferase reporter assays. Results: We observed that PIK3CA was ... «Dove Medical Press, 七月 15»
Parasitology: CRISPR for Cryptosporidium
The authors verified successful transfections using a marker gene that encodes the protein luciferase, which produces bioluminescence in the ... «Nature.com, 七月 15»
Designer gut bacteria could help fight stomach diseases in the future
To do that, they used a technique called bacterial conjugation to insert a gene called luciferase that codes for fluorescence into the gut ... «Genetic Literacy Project, 七月 15»
PhaseRx Demonstrates Therapeutic Efficacy of mRNA Therapeutics …
Through the application of its Hybrid mRNA Delivery System™, PhaseRx has demonstrated high-level expression of the luciferase reporter ... «PipelineReview.com, 七月 15»
Wnt/β-catenin signaling regulated SATB1 promotes colorectal …
Next we cloned 381 bp of human Satb1 promoter in Luciferase reporter vector and analyzed activity of Satb1 promoter upon β-catenin ... «Nature.com, 七月 15»
